Journal: Cell Death Discovery
Article Title: NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving
doi: 10.1038/s41420-021-00521-0
Figure Lengend Snippet: A The relative mRNA levels of MKP2 and MKP7 in NC and OV cells were determined with qPCR. B and C The relative MKP2 and MKP7 protein levels in both OV and NC cells were determined with Western Blotting. D The protein levels of MKP7 and NR4A1 in response to 100 μM H 2 O 2 in NC cells. E – H The protein levels of MKP7 in response to 100 μM H 2 O 2 and 0.5 μM TG at various time points in both OV and NC cells. The data represented the means of three independent experiments, * P < 0.05, ** P < 0.01 vs. ns. Error bars were shown as SD values.
Article Snippet: The antibodies used in the experiment were as follows: cleaved caspase-3 Antibody (9661) and rabbit anti-pJNK antibody (4668) were purchased from CST; mouse anti-GAPDH (10494-1-AP), rabbit anti-MKP2 antibody (10739-1-AP), and rabbit anti-MKP7 antibody (14237-1-AP) were derived from Proteintech Group (Wuhan, China); rabbit anti-NR4A1 antibody (ab13851 and DF7850) was purchased from Abcam and Affinity; mouse anti-β-Actin (BS6007M) and rabbit anti-tubulin α (BS1699) were purchased from Bioworld Technology.
Techniques: Western Blot